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fusA [2019-06-28 12:42:29]
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fusA [2019-06-28 12:42:29]

elongation factor G, facilitates movement of tRNA and mRNA by one codon
locus
BSU01120
pI
4.61
mw
76.36 kDa
protein length
692 aa Sequence Blast
gene length
2079 bp Sequence Blast
function
translation
product
elongation factor G
essential
yes
synonyms
fus
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • Gene

    Coordinates
    130,684 132,762

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • hydrolyses GTP

    • Protein family

  • [SW|TRAFAC class translation factor GTPase superfamily] (according to UniProt)

    • Modification

  • phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [Pubmed|17218307], [Pubmed|17726680]
  • phosphorylated on Tyr-339 [Pubmed|26381121]

    • Structure

  • [PDB|1ELO] (from ''Thermus thermophilus'') [Pubmed|8070397]
  • [PDB|4BTC], [PDB|4BTD], the ''Thermus thermophilus'' EF-G[SW|ribosome] complex in a pretranslocation state [Pubmed|23912278]

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

    • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the mRNA is processed between [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] and [gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Expression vectors

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP840, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP848, available in [SW|Jörg Stülke]'s lab

    • References

  • 24614227,17726680,17218307,20348921,8070397,23912278,15378759,25108354,26381121,27524615,26072700